BS ISO 27447:2019 pdf free.Fine ceramics (advanced ceramics, advanced technical ceramics)一Test method for antibacterial activity of semiconducting photocatalytic materials.
This document is for the development, comparison, quality assurance, characterization, reliability and design data generation of photocatalytic materials. The method is used to obtain the antibacterial activity of photocatalytic materials by the contact of a specimen with bacteria, under UV light irradiation. The film cover method is available for flat sheet, board or plate-shaped materials. To avoid warpage in the cloths or textiles, the glass cover method is available for the cloths or textiles. The specimen is laid in a Petri dish and the bacterial suspension is dripped onto the specimen. Then the cover film or glass is placed on the suspension and the moisture conservation glass is placed on top of the Petri dish. The Petri dish containing the specimen is exposed to light. After exposure, the test bacteria are washed out of the specimen and the cover film or glass. This washout suspension is measured by the viable bacterial count method. The results obtained are compared with those obtained from inoculated specimens of non-photocatalytic treated material exposed to UV irradiation under identical conditions to the treated material, and to those obtained from inoculated specimens of both photocatalytic treated and non-treated material kept in the dark for the same period of time.The bacteria strains to be used in the test are equivalent to those described in Table 1 and are stored by entities that are registered under the World Federation for Culture Collections or the Japan Society for Culture Collections. Aseptic manipulations using microorganisms can be performed in an adequate safety cabinet. Inoculate each strain into a slant culture medium (nutrient agar medium), incubate for 16 h to24 h at37°C+ 1 °C, and then store in a refrigerator at5 °C to 10 °C. Repeat subcultures within 1 month by replicating this process. The maximum number of subcultures from the original strain transferred by culture collection is 10 times. In the case of bacteria stored in a deep freezer, the maximum number of subcultures from the original strain transferred by culture collection is 10. The slant culture shall not be used for further storing after 1 month.BS ISO 27447 pdf download.