BS EN 1276:2019 pdf free.Chemical disinfectants and antiseptics一Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas一Test method and requirements (phase 2, step 1). The product shall demonstrate at least a 5 decimal logarithm (g) reduction (3 Ig for handwashes) when diluted with hard water (5.2.2.7) or- in the case of ready-to-use products – with water (5.2.2.2) and tested in accordance with Clause 5 under simulated clean conditions [0,3 g/l bovine albumin solution-5.2.2.8.2) or simulated dirty conditions (3 g/l bovine albumin solution – 5.2.2.8.3) according to its practical applications and under the suitable test conditions as described in 5.5.1.1, Tables 1 and 2 here below. Where indicated, additional specific bactericidal activity shall be determined applying other interfering substances and test organisms (in accordance with 5.2.1, 5.2.2.8 and 5.5.1.1) in order to take into account intended specific use conditions.A sample of the product as delivered and/or diluted with hard water (or water for ready-to-use products with the exception of handwash products whose first dilution is done in hard water (5.4.2]) is added to a test suspension of bacteria in a solution of an interfering substance. The mixture is maintained at the chosen test temperature for the adopted contact time. At the end of this contact time, an aliquot is taken, and the bactericidal and/or the bacteriostatic activity in this portion is immediately neutralized or suppressed by a validated method. The method of choice is dilution-neutralization. If a suitable neutralizer cannot be found, membrane filtration is used. The numbers of surviving bacteria in each sample are determined and the reduction is calculated.The test is performed using Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus hirae as test organisms. For temperatures≥40 °C only Enterococcus faecium shall be used.If additional test organisms are used, they shall be incubated under optimum growth conditions (temperature, time, atmosphere, media) noted in the test report. If the additional test organisms selected do not correspond to the specified strains, their suitability for supplying the required inocula shall be verified. If these additional test organisms are not classified at a reference centre, their identification characteristics shall be stated. In addition, they shall be held by the testing laboratory or national culture collection under a reference for five years.BS EN 1276 pdf download.