BS EN ISO 20743:2007 pdf.Textiles – Determination of antibacterial activity of antibacterial finished products.
These lest methods were established in order to address the substantial need for an International Standard to determine antibacterial activity for antibacterial finished textile products.
This International Standard specifies quantitative test methods to determine the antibacterial activity of antibacterial finished textile products including nonwovens.
This International Standard is applicable to all textile products, including cloth, wadding, thread and material for clothing, home furnishings and miscellaneous goods regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).
Based on the intended application and on the environment in which the textile product is to be used, the user can select the most suitable of the following three methods on determination of antibacterial activity:
a) absorption method (an evaluation method in which test bacterial suspension is inoculated directly onto samples);
b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto samples);
c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto samples).
The colony plate count method and the ATP (ATP Adenosine Tn-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.
4 Safety precaution
Test methods specified herein require the use of bacteria.
These tests should be carried out by persons with training and experience in the use of microbiological techniques.
Appropriate safety precautions should be observed with due consideration given to country-specific regulations.
5 Apparatus
Usual laboratory apparatus and, in particular, the following.
5.1 Spectrophotometer, capable of measuring at a 620 nm to 660 nm wavelength, or McFarland’s nephelometer.
5.2 Incubator, capable of maintaining a constant temperature of 37 °C ± 2 °C.
6 Reagents and culture media
Reagents used in tests shall be of analytical quality and/or suited for microbiological purposes.
Dehydrated products available on the commercial market are recommended for use in preparing the culture media. The manufacturer’s instructions for the preparation of these products should be strictly followed.
6.1 Water.
Water used in tests shall be analytical-grade water for microbiological media preparation which is freshly distilled and/or ion-exchanged and/or ultra-filtered and/or filtered with RO (reverse osmosis). It shall be free from all toxic or bacteria inhibitory substances.BS EN ISO 20743:2007 pdf free download.