BS ISO 22992-2:2020 pdf free.Textiles一Determination of certain preservatives Determination of triclosan residues method using LC-MS/MS.
BS ISO 22992-2 1 Scope This document specifies a method for determination of triclosan residues in textiles by high performance liquid chromatography一tandem mass spectrometry (HPLC-MS/ MS). This method is applicable to all kinds of textile products. 2 Normative references The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 3696, Water for analytical laboratory use一Specification and test methods.The triclosan is extracted from textile specimen by ultrasonic generator with methanol. After being concentrated and diluted to volume, the residue is determined by HPLC-MS/MS, quantified by external standard method. Statistical data of interlaboratory trial is given in Annex B.7.1.2 Working solution Prepare a working solution of the triclosan in methanol and dilute it to a series of suitable concentrations depending on test needs. Select at least five appropriate dilutions of the calibration sets to create calibration curve. NOTE Standard stock solution is kept in the refrigerator at 0 °C to 4 °C for up to 6 months. Working solution is kept in the refrigerator at 0 °C to 4 °C for up to 3 months. 7.2 Preparation of test specimen Prepare a representative test specimen of the sample. Cut it into small pieces with a maximum dimension less than 5 mm and mix them homogeneously. Weigh (1,00 土0,01) g of the pieces with a balance 6.5).7.3 Ultrasonic wave extraction Put the pieces into a vial with tight closure (6.7) and add 25 ml of methanol (5.2). Place the vial in an ultrasonic generator (6.3) at about 40 °C for (30士2) min. Filter and transfer the extract into 100 ml flask (6.8]. Add 25 ml of methanol to the residue and place the vial in the ultrasonic generator to extract the residue at about 40 °C for (30士2) min. Filter and merge the extract into the flask [6.8].BS ISO 22992-2 pdf download.