BS ISO 23905:2020 pdf free.Cigarettes – Determination of selected phenolic compounds in cigarette mainstream smoke using HPLC-FLD.
BS ISO 23905 This document specifies a method for the quantification of selected phenolic compounds by high performance liquid chromatography with fluorescence detection (HPLC-FLD) using ISO 3308 smoking parameters. The selected phenolic compounds are: hydroquinone, resorcinol, catechol, phenol, p-Cresol,m-Cresol and o-Cresol.This method is applicable to cigarettes with total particulate matter (TPM) yields between 1 mg/cigarette and 16 mg/cigarette.The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 3308, Routine analytical cigarette-smoking machine – – Definitions and standard conditions ISO 3402, Tobacco and tobacco products一Atmosphere for conditioning and testing ISO 4387, Cigarettes一Determination of total and nicotine-free dry particulate matter using a routine analytical smoking machine ISO 8243, Cigarettes一Sampling.After all samples have been smoked following ISO 3308, remove the glass fibre filter pad from the smoking machine, fold into quarters and place into a 125 ml extraction flask for 44 mm glass fibre filter pad (or in a 250 ml extraction flask for 92 mm glass fibre filter pad]. Add 40 ml of 1 % acetic acid solution (7.2) for 44 mm glass fibre filter pad [80 ml for 92 mm glass fibre filter pad). Cover the flask with ground glass stopper, shake the flask until the glass fibre filter pad has disintegrated and filter the extract through a 0,45 μum syringe filter.
NOTE  There might be a need to dilute (with 1 % acetic solution) the obtained solution so that the concentration of phenolic compounds is within the calibration range.Transfer an aliquot of the filtered extract to a vial and fill the vial to minimize the headspace.Analyse each calibration standard (see 7.3.3). Record the area, generate a calibration curve using a linear function. Calculate a response factor for each individual phenolic compound. The response obtained for test samples shall fall within the working range of the calibration curve.The concentration of each phenolic compound in cigarette mainstream smoke samples is quantified by the external standard method. The identification of each phenolic compound peak in samples is
determined by its comparison with the respective retention times in standards.BS ISO 23905 pdf download.